Recombinase Polymerase Amplification and Their Application in Phytopathogen Detection
DOI:
https://doi.org/10.33102/mjosht.v8i2.254Keywords:
isothermal amplification, recombinase polymerase amplification, plant pathogens, diagnosticAbstract
DNA identification method is indispensable for the detection of a plant pathogen. However, established techniques, though reliable, requires advanced equipment, and their application outside specialized laboratories is limited. Along with the advancement of molecular techniques, several isothermal amplification methods, including Recombinase Polymerase Amplification (RPA), has been developed in this study. In fact, RPA is a rapid and sensitive amplification method, operating optimally at 37-42 degree celcius for 15 to 30 minutes with minimal sample preparation, and can amplify as low as 1-10 target copies. Furthermore, RPA has been a favourable method for the detection of plant pathogens due to its advantageous parameters. This review presents the current knowledge of RPA and its application in plant pathogen detection.
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