An In Vitro Study Of Antibacterial Efficacy Of Salvadora Persica (Miswak) Extract Against Enterococcus Faecalis

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  • Munirah Che Rosli Department of Biomedical Science, International Islamic University, Kulliyyah of Allied Health Sciences, Kuantan, 25200, Pahang.
  • Intan Azura Shahdan Department of Biomedical Science, International Islamic University, Kulliyyah of Allied Health Sciences, Kuantan, 25200, Pahang.
  • Dr Mohd Haikal Muhamad Halil Department of Restorative Dentistry, International Islamic University, Kulliyyah of Dentistry, Kuantan, 25200, Pahang.



root canal, Enterococcus faecalis, Salvadora persica, sodium hypochlorite


Salvadora persica is widely used in dental field to improve dental health and promote dental hygiene. Although sodium hypochlorite is highly recommended and regarded as the most effective irrigant in root canal treatment to eliminate bacteria, it can cause harm to patients manifested by severe pain, rapid swelling and also obstruct breathing airway. However, it is believed that the chemical composition of Salvadora persica helps to treat and act as an antibacterial agent in root canal therapy. This study aims to obtain the crude compound from Salvadora persica by using the freeze drying method and to compare the antibacterial activity of the Salvadora persica and sodium hypochlorite against Enterococcus faecalis in aerobic condition. Antibacterial activity results showed that 150 mg/ml of Salvadora persica give comparable effect with sodium hypochlorite against Enterococcus faecalis. It is believed that 60% of alcoholic Salvadora persica extraction is an effective antimicrobial agent to be utilized as an irrigant in root canal treatment.


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Abo Al – Samh D. Al- Bagieh N (1996) . A study of Antibacterial activity of the Salvadora persica extract in vitro. Biomedical Letters53: 225-238 doi: 10.1155/2016/7083964

Akhilanand Chaurasia Ranjit Patil, Amit Nagar (2013). A Review Article, Salvadora persica in Oral Cavity - An Update, Journal of Oral Biology and Craniofacial Research 3 pp. 98-101 doi: 10.1016/j.jobcr.2012.09.004

Article, Salvadora persica in Oral Cavity - An Update, Journal of Oral Biology and Craniofacial Research 3 pp. 98-101 doi: 10.1016/j.jobcr.2012.09.004

Akhtar MS, Ajmal M.Significance of Chewing Sticks (Salvadora Persica) in oral hygiene from pharmacological viewpoint. Journal of Pakistan Medical Association. 2000:4-84-95 doi: 10.4103/1305-7456.178297

Al-Bayati, F., Sulaiman,K., (2008). In vitro Antimicrobial Activity of Salvadora Persica Extracts Against Isolated Oral Pathogens in Iraq, Turk Journal Biology, 32.pp.57-62. doi: 10.1155/2016/7083964

Al-Bageih, N., Idowu, A., Salako, N.O., (1994). Effect of Aquours Extract of Salvadora persica on the in vitro Growth of Candida albicans Microbios, 80(323), pp. 107-113.

Al-Lafi T., Ababneh H., (1995), The Effect of the Extract of the Salvadora persica (chewing sticks) used in Jordan and the Middle East on oral bacteria. International Dental Journal, 45, pp. 218-222.

Al-Mas, Khalid, (2001). The Antimicrobial Effects of Seven Different Types of Asia Chewing Sticks. Odonto-Stomalogie Tropicale Journal, 24(96), pp. 17-20.

Almas K., Al-BAgieh, N. Akpata, E., (1997). In vitro Antimicrobial Effects of Freshly Cut and 1 month old Salvador persica (chewing stick). Biomedical. Lett, 56, pp.145-149. doi: 10.12669/pjms.302.4284

Amir Moeintaghavi, Hamidreza Arab, Mehrangiz khajekaramodini, Rohollah Hosseini Hossein Danasteh, Hamed Niknami (2012). In vittra Antibacterial Comparison of Chlorhexidine, Persica Mouthwash and Salvadora persica Extract. Journal of Contemporary Dental Practice, 13(2), pp. 147-152. doi: 10.5005/jp-journals-10024-1111

Assadi SG, Asadi ZG. Chewing sticks and oral hygiene habits of the adult Pakistani population. Int Dent J. 1997; 47:275-8 doi: 10.1002/j.1875-595X.1997.tb00789.x

A.T.Khalil, Benzylamides from Salvadora persica, Archives Pharmacal Research, 29, pp 952-956.

Azhal Iqbal (2012). International Journal of Health Sciences, Antimicrobial Irrirgants in Endodontic Therapy, International Journal Health Sciences (Qassim), 6(2), pp.182-192

Bayaty F., Abdulla M, Hassan M, Roslan S, Hussain S, Said H (2010). Effect of mouthwash extracted from Miswak (Salvadora persica) on periodontal pathogenic bacteria an in vitro study. Science and Social Research, 4:p178-810. doi: 10.5897/JMPR10.198

Bystrom G, Sundaqvist G, The Antibacterial Action on Sodium Hypochlorite and EDTA in 60 cases of Endodontic Therapy. International Endodontic, 18, pp.35-40. doi: 10.1111/j.1365-2591.1985.tb00416.x

Daurot I, Albandar, J., Skaung N., Ali R., (2002). Salivary Microbiota Level in Relation to Periodontal Status, Experience of Caries and Salvadora persica use in Sundanese Adults. Journal Clinical Periodontal, 29(5), pp.411-420. doi: 10.1034/j.1600-051X.2002.290505.x

Dominic Peter Laverty (2014), Sodium Hypochlorite Accident During Endodontic Treatment, Journal of Restorative Dentistry vol 2 pp 94-100 doi: 10.16966/2378-7090.114

Estrela C, Silva JA de Alencer AH, Leles CR, Decurcio DA (2008), Efficacy of sodium hypochlorite and Chlorhexidine Against Enterococcus faecalis: A systemic review. Journal Applicato Oral Science, 16:364 doi: 10.1590/s1678-77572008000600002

Fouad Hussein Al-Bayaty, Aiman Hamad Al-Koubaisi, Nidhal Abdul Wahid Ali, Mahmood Ameen Abdulla (2010). Effect of Mouthwash extracted from Salvadora persica on dental plaque formation: A clinical trial. Journal of Medical Plants Research, 4(14) pp 1446-1454. doi: 10.5897/JMPR10.198

Gatot A, Arbelle J, Leiberman A, Yanai-Inbar I (1991) Effect of Sodium Hypochlorite on soft tissues after inadvertent injection beyond root apex. Journal of Endodontics:7-573-4 doi: 10.1016/S0099-2399(06)81725-5

Gerhardt CR, Eppendorf K, Kozlowski A, Brandt M (2004), Toxicity of Concentrated Sodium Hypochlorite used an Endodontic Irrigant. International Endodontic Journal 37:272-280 doi: 10.1111/j.0143-2885.2004.00804.x

Guile EE, Al-Shammery, AR, El-Backly MN. Oral health survey of Saudi Arabia: Oral Health Knowledge Attitude and Practice Among Adults. Journal Dentistry Research 1996:75 doi: 10.4236/health.2010.25074

Hassan Sulaiman Hallway (2012). A Review on Salvadora persica and Its Effect on Various Aspect of Oral Health. The Saudi Dental Journal, 24, pp.63-69. doi: 10.1016/j.sdentj.2011.12.004

Hales JJ, Jackson CR, Everett AP. Moorre SH (2001). Treatment Protocol for the Management of a Sodium Hypochlorite Accident During Endodontic Therapy, General Dentistry 49:278-281

Hulsmann M, Hahn W., (2000). Complications during Root Canal Irrigation - Literature Review and Case Report. International Endodontic Journal, 33(3), pp 186-193. doi: 10.1046/j.1365-2591.2000.00303.x

Khalessi AM, Pack AR, Thomson WM, Tompkins GR. Extracts of Salvadora persica. Int Dent J. 2004;54:279-283

Maisa o Al-Sebaei, Omar A Halabi, Ibrahim E El-Hakim (2015). Sodium Hypochlorite Accident resulting in life threatening airway obstruction during root canal treatment: a case report. Clinical Cosmetic and Investigational Dentistry 7, pp.41-44. doi: 10.2147/CCIDE.S79436

Mandel ID (1988). Chemotherapeutic Agents for Controlling Plaque and Gingivitis. Journal Clinic Periodontal 15, pp. 488-498. doi: 10.1111/j.1600-051X.1988.tb01020.x

Mansour MI, Al-Khateeb TL and Al-Mazraoo AA., (1996). The Analgesic Effect of Salvadora persica. Saudi Dental Journal, 8, pp.87-91.

Mark D Essner, Amjad Javed, Paul D Eleazer (2011), Oral Surgical Oral Med Oral Radiol Endod. Effect of Sodium HYpochlorite on human pulp cells : an vitro study, 112(5):662-666 doi: 10.1016/j.tripleo.2011.04.030

Mumghamba EG, Manji KP, Michael J. Oral hygiene practices, periodontal conditions, dentition status and self-reported bad mouth breathe among young mothers, Tanzania. Int J Dent Hyg. 2006;4:166-173. doi: 10.1111/j.1601-5037.2006.00186.x

Nordin Fatin - Majdina, Haji Abd Rahim, Abd Razak Monica @ MUnirah, Mohd Bakri MArina (2014), Sains Malaysiana, Effect of Salvadora persica Extract on the bacterial population in single-species Biofilm, 43(12): 1889-1893. doi: 10.17576/jsm-2014-4312-10

Orstaik D, Haapasalo M, (1990). Disinfection by endodontic, irritants and dressing of experimentally infected dentinal tubules. Endodontic Dental Traumatol, 6 p.p. 142-149 doi: 10.1111/j.1600-9657.1990.tb00409.x

Qian-QIan Wang, Cheng - Fei Zhang, Chun - Hung Chu, Xiao - Fei Zhu (2012). Prevalence of Enterococcus faecalis in saliva and filled root canals 0f teeth associated with apical periodontitis, International Journal of Oral Science, 4, pp. 19-20. doi: 10.1038/ijos.2012.17

Raed I, Al-Sadhan, Khalid Almas, (1999). Salvadora persica (Chewing stick): A cultural and Scientific Heritage. The Saudi Dental Journal, 11, 2.

Shamat A. M:Babiker I.A: Mukhtar M.S: Ahmed F.A (2010). A study of the seasonal and regional variations in nutritive value of some important plant species selected by camels in arid and semi-arid lands (ASAL) of Sudan. Journal Applied Science. Restoration, 6(8) 1265-1272.

Shabahang S, Torabinejad M (2003), In vitro antibacterial on Enterococcus faecalis contaminated root canals of extracted human teeth. Journal Endodontic, 29, pp.576-579.

Talal H A-l-Salman, Moataz Gh Al-Shaekh Ali, Osama M Al-Nu’aimy (2005). The antimicrobial effect of water extraction of Salvadora persica as root canal irrigant. Al-Rafidain Dental Journal, 5, 1, pp. 33-36. doi: 10.33899/rden.2005.45508

Walid H.Foud H (2001). The Effect of Myrtus Communes Extract mouth washing newly dental plaque. Journal of American College Dentistry, 8 pp. 41-49.

Zhang W, Torabinejad M, Liy (2003). Evaluation of cytotoxicity of MTAD using the MTT-tetraolium method. Journal endodontic 29, pp.654-657. doi: 10.1097/00004770-200310000-00010

British Dental Association (December, 2014), BDA Evidence Summary, Irrigation in Endodontics. Pp 2-8. doi: 10.1038/sj.bdj.2014.204

B. Gomes, E.T.Pinheiro, (2004). Microbiological Examination of Infected Dental Root Canals Oral Microbiology Immunology, 19, pp. 71-76. doi: 10.1046/j.0902-0055.2003.00116.x

Ercan E, Dalli M, Yavuz, Ozekinci T. (2006), Investigation of Microorganism In Infected Dental Root Canals, Biotechnol & Biotechnol pp166-172. doi: 10.1080/13102818.2006.10817361

Lewis WH, Elvin-Lewis (1997) MPF. Oral Hygiene Medical Botany, John Wiley & Sons, New York, 226-270.

Nannin, E. C., & Murray, B.E. (2006). Enterococcus spp. In S.H. Gillespie, & P.M. Hawkey (Eds.), Principles and Practice of Clinical Bacteriology (2nd ed., pp. 59-71). West Sussex UK: John Wiley & Sons, Ltd.

Orwa C, A Mutua, Kindt R, Jamnadass R, S Anthony., (2009). Agroforestree Database: a tree reference and selection guide version 4.0

Public Health Agency of Canada, (2010). Enterococcus faecalis: Pathogen safetydatasheet - Infectious substances.

Teixeira, L.M., Carvalho, Maria da Gloria Siqueira & Facklam, R.R.(2007). Enterococcus In P.R.Murray (Ed.), Manual of Clinical Microbiology, 9, p.p. 430-442.

World Health Organization (1987) Prevention of Oral Disease. Geneva:WHO


DOI: 10.33102/mjosht.v8i1.225
Published: 2022-02-07

How to Cite

Munirah Che Rosli, Intan Azura Shahdan, & Dr Mohd Haikal Muhamad Halil. (2022). An In Vitro Study Of Antibacterial Efficacy Of Salvadora Persica (Miswak) Extract Against Enterococcus Faecalis . Malaysian Journal of Science Health & Technology, 8(1), 55–62.