Used Palm Cooking Oil as Substrate for Optimization of Lipase Activity in Aeromonas caviae SS-2

Azarul Afandi Suhaili; Siti Salhah Othman; Ismatul Nurul Asyikin Ismail; Aswath Rama Reddy

doi:10.33102/mjosht.559

Authors

Azarul Afandi Suhaili Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
Siti Salhah Othman Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
Ismatul Nurul Asyikin Ismail Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
Aswath Rama Reddy Department of Microbiology, Periyar University, Periyar, Palkalai Nagar, Tamil Nadu 636011, India.

DOI:

https://doi.org/10.33102/mjosht.559

Keywords:

Isolation, lipase, environmental pollution, substrate, used palm cooking oil

Abstract

Palm oil is widely used for culinary applications, industrial formulations, and oleochemical processing. The increasing demand for palm oil for household and industrial use indirectly contributes to environmental pollution, particularly through the unethical disposal of palm oil waste. In this study, a sustainable biotechnological approach is explored to utilize this waste by exploiting the catalytic potential of bacterial lipases. Lipase-producing bacteria have been isolated from used palm oil-contaminated environments in Nilai, Negeri Sembilan, using the stab agar screening method. Among 90 potential isolates, Aeromonas caviae SS-2 demonstrates the highest lipase specific activity at 9.189 µmol/mL/min within just 9 hours of incubation at 40℃. A. caviae SS-2 indicates an optimum lipase production when it is cultivated in Tryptone Azolectin Tween (TAT) broth supplemented with 1% (v/v) of Tween 40 and 2% (v/v) of used palm oil. This enzymatic biotransformation efficiently hydrolyzed long-chain triglycerides into shorter, value-added carbon chains, demonstrating its potential for lipid production and industrial bio-conversion.

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