Identification of Antioxidants and Active Compounds in Cow’s Milk

Abstract

This paper aims to analyze and identify the antioxidant compounds in cow’s milk. Milk contains high biological molecules protein, essential fatty acids, calcium, fat, amino acid, vitamins that are soluble in water and several bioactive compounds. In Al-Quran and Hadith, milk has been mentioned several times especially for breast feeding which is essential for infant immunity. Antioxidant can suppress free radicals and unstable molecules produced by the body as an environmental and other pressure reaction. Therefore, this study focuses on the identification of antioxidant compounds in milk sample of Dutch Lady Fresh Milk and Goodday Fresh Milk using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR). Based on the ATR-FTIR spectrum, we are able to identify the functional groups of antioxidant compounds which are C–H stretch of alkanes, C=O of aldehydes, C–N stretch of aliphatic amine, C–Cl stretch of alkyl halide, O–H bend of carboxylic acids and N–H wag of 1 , 2 amines. This is the first research that studied on the antioxidant compounds in milk sample of Dutch Lady Fresh Milk and Goodday Fresh Milk. Further, the characterization of antioxidant compounds will be perform using Gas Chromatography-Mass Spectrometry (GC-MS) analysis.